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Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli.

Price WN, Handelman SK, Everett JK, Tong SN, Bracic A, Luff JD, Naumov V, Acton T, Manor P, Xiao R, Rost B, Montelione GT, Hunt JF,
Microbial informatics and experimentation (2011) 1:6 PublishedPSI:Biology  
Northeast Structural Genomics Consortium

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The biochemical and physical factors controlling protein expression level and solubility in vivo remain incompletely characterized. ...
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