|Structure (2011) 19:1885-94|
|Northeast Structural Genomics Consortium|
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Bacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. ...
The phiKZ virion consists of a 1450 Å diameter icosahedral head and a 2000 Å-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 Å resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 Å resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4.
|metabolism chemistry |
|Crystallography, X-Ray Protein Conformation Protein Folding Microscopy, Electron Bacteriophage T4 Myoviridae Viral Tail Proteins |
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