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High-throughput protein purification and quality assessment for crystallization.

Kim Y, Babnigg G, Jedrzejczak R, Eschenfeldt WH, Li H, Maltseva N, Hatzos-Skintges C, Gu M, Makowska-Grzyska M, Wu R, An H, Chhor G, Joachimiak A,
Methods (2011) 55:12-28 PublishedPSI:Biology  
Center for Structural Genomics of Infectious DiseasesMidwest Center for Structural Genomics

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The ultimate goal of structural biology is to understand the structural basis of proteins in cellular processes. ...
metabolism chemistry methods genetics 
Crystallography, X-Ray Humans Escherichia coli Proteomics Chromatography, Gel Crystallization Protein Folding Recombinant Proteins Magnetic Resonance Spectroscopy Chromatography, Affinity Endopeptidases Automation, Laboratory High-Throughput Screening Assays 
21907284  
10.1016/j.ymeth.2011.07.010  
69 (Last update: 10/20/2018 1:38:04pm)  
In situ proteolysis
Large-scale evaluation of protein reductive methylation
New vectors for co-expression of proteins
Crystal production
LIC (Ligation-Independent Cloning) vectors
Protein production
Target selection
Cleavable C-terminal His-tag vectors for structure determination
New surface contacts formed upon reductive lysine methylation: improving the probability of protein crystallization
PDPredictor: Predicting crystallizability from protein sequence
Fermentation for large-scale production of native and isotopically enriched proteins
Protein Characterization
Gene amplification and cloning
 
method