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Efficient condensed-phase production of perdeuterated soluble and membrane proteins.

Schneider WM, Tang Y, Vaiphei ST, Mao L, Maglaqui M, Inouye M, Roth MJ, Montelione GT,
Journal of Structural and Functional Genomics (2010) 11:143-54 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Protein perdeuteration approaches have tremendous value in protein NMR studies, but are limited by the high cost of perdeuterated media. ...
metabolism chemistry genetics 
Escherichia coli Escherichia coli Proteins Nuclear Magnetic Resonance, Biomolecular Heat-Shock Proteins Multienzyme Complexes Membrane Proteins Bacterial Outer Membrane Proteins Gene Expression Genetic Vectors Deuterium Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization 
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