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Efficient condensed-phase production of perdeuterated soluble and membrane proteins.

Schneider WM, Tang Y, Vaiphei ST, Mao L, Maglaqui M, Inouye M, Roth MJ, Montelione GT,
Journal of Structural and Functional Genomics (2010) 11:143-54 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Protein perdeuteration approaches have tremendous value in protein NMR studies, but are limited by the high cost of perdeuterated media. ...
chemistry genetics metabolism 
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Escherichia coli Genetic Vectors Deuterium Multienzyme Complexes Membrane Proteins Bacterial Outer Membrane Proteins Nuclear Magnetic Resonance, Biomolecular Heat-Shock Proteins Escherichia coli Proteins Gene Expression 
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