View publication

Independently inducible system of gene expression for condensed single protein production (cSPP) suitable for high efficiency isotope enrichment.

Schneider WM, Inouye M, Montelione GT, Roth MJ,
Journal of Structural and Functional Genomics (2009) 10:219-25 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

(click to unfold)
The ability to produce isotope-enriched proteins is fundamental to the success of modern protein NMR, and is particularly essential for NMR activities in structural genomics projects. ...
genetics metabolism 
Isopropyl Thiogalactoside Escherichia coli Genetic Vectors Molecular Sequence Data Cloning, Molecular Isotope Labeling Base Sequence Proteins Nuclear Magnetic Resonance, Biomolecular Gene Expression 
19642019  
10.1007/s10969-009-9067-x  
9 (Last update: 03/25/2017 12:10:07pm)  
Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system
 
method