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Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.

Goren MA, Fox BG,
Protein Expression and Purification (2008) 62:171-8 PublishedPSI:Phase 2  
Center for Eukaryotic Structural Genomics

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A wheat germ cell-free extract was used to perform in vitro translation of human stearoyl-CoA desaturase in the presence of unilamelar liposomes, and near complete transfer of the expressed integral membrane protein into the liposome was observed. ...
metabolism genetics enzymology isolation & purification biosynthesis analysis drug effects pharmacology 
Humans Animals Molecular Sequence Data Catalysis Liposomes Mice Base Sequence Genetic Vectors Protein Biosynthesis Isoenzymes Detergents Cell-Free System Triticum Mycobacterium Cytochromes b5 Electrophoresis, Capillary Plant Extracts Stearoyl-CoA Desaturase 
18765284  
10.1016/j.pep.2008.08.002  
54 (Last update: 07/14/2018 5:07:07pm)  
Activity and topology determination of in vitro expressed membrane proteins
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex
 
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