|Nature Methods (2008) 5:135-46|
|Integrated Center for Structure and Function InnovationJoint Center for Structural GenomicsMidwest Center for Structural GenomicsNortheast Structural Genomics ConsortiumNew York Structural Genomics Research Consortium|
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In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. ...
To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
|methods isolation & purification metabolism |
|Recombinant Proteins Chemistry, Physical Proteomics Chemical Fractionation Protein Engineering |
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