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Microgram-scale protein structure determination by NMR.

Aramini JM, Rossi P, Anklin C, Xiao R, Montelione GT,
Nature Methods (2007) 4(6):491-3 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. ...
chemistry methods 
Models, Molecular Archaeal Proteins Methanosarcina Magnetic Resonance Spectroscopy Protein Conformation 
17496898  
10.1038/nmeth1051  
30 (Last update: 03/18/2017 11:29:57am)  
Microscale Protein NMR Structure Determination
Microscale protein NMR structure determination
 
method  
Nat Methods. 2007 Jun;4(6):491-3. Epub 2007 May 13.