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Microgram-scale protein structure determination by NMR.

Aramini JM, Rossi P, Anklin C, Xiao R, Montelione GT,
Nature Methods (2007) 4(6):491-3 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. ...
chemistry methods 
Models, Molecular Archaeal Proteins Methanosarcina Magnetic Resonance Spectroscopy Protein Conformation 
31 (Last update: 01/11/2018 2:33:04pm)  
Microscale Protein NMR Structure Determination
Microscale protein NMR structure determination
Nat Methods. 2007 Jun;4(6):491-3. Epub 2007 May 13.