|Nature Methods (2007) 4(6):491-3|
|Northeast Structural Genomics Consortium|
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Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. ...
This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
|chemistry methods |
|Models, Molecular Archaeal Proteins Methanosarcina Magnetic Resonance Spectroscopy Protein Conformation |
|31 (Last update: 11/09/2017 4:09:09pm)|
Microscale Protein NMR Structure Determination
Microscale protein NMR structure determination
|Nat Methods. 2007 Jun;4(6):491-3. Epub 2007 May 13.|