|Journal of Biological Chemistry (2007) 282(28):20584-92|
|Northeast Structural Genomics Consortium|
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Influenza A viruses cause a highly contagious respiratory disease in humans and are responsible for periodic widespread epidemics with high mortality rates. ...
The influenza A virus NS1 protein (NS1A) plays a key role in countering host antiviral defense and in virulence. The 73-residue N-terminal domain of NS1A (NS1A-(1-73)) forms a symmetric homodimer with a unique six-helical chain fold. It binds canonical A-form double-stranded RNA (dsRNA). Mutational inactivation of this dsRNA binding activity of NS1A highly attenuates virus replication. Here, we have characterized the unique structural features of the dsRNA binding surface of NS1A-(1-73) using NMR methods and describe the 2.1-A x-ray crystal structure of the corresponding dsRNA binding domain from human influenza B virus NS1B-(15-93). These results identify conserved dsRNA binding surfaces on both NS1A-(1-73) and NS1B-(15-93) that are very different from those indicated in earlier "working models" of the complex between dsRNA and NS1A-(1-73). The combined NMR and crystallographic data reveal highly conserved surface tracks of basic and hydrophilic residues that interact with dsRNA. These tracks are structurally complementary to the polyphosphate backbone conformation of A-form dsRNA and run at an approximately 45 degrees angle relative to the axes of helices alpha2/alpha2'. At the center of this dsRNA binding epitope, and common to NS1 proteins from influenza A and B viruses, is a deep pocket that includes both hydrophilic and hydrophobic amino acids. This pocket provides a target on the surface of the NS1 protein that is potentially suitable for the development of antiviral drugs targeting both influenza A and B viruses.
|chemistry mortality pathogenicity metabolism |
|Crystallography, X-Ray Protein Structure, Quaternary Humans Dimerization Influenza A virus Nucleic Acid Conformation Influenza, Human RNA, Viral Protein Folding Protein Structure, Secondary Viral Nonstructural Proteins Nuclear Magnetic Resonance, Biomolecular Protein Binding RNA, Double-Stranded Influenza B virus |
|65 (Last update: 04/01/2017 11:36:35am)|
|J Biol Chem. 2007 Jul 13;282(28):20584-92. Epub 2007 May 1.|