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Bacterial bioreactors for high yield production of recombinant protein.

Suzuki M, Roy R, Zheng H, Woychik N, Inouye M,
Journal of Biological Chemistry (2006) 281(49):37559-65 PublishedPSI:Phase 2  
New York Consortium on Membrane Protein StructureNortheast Structural Genomics Consortium

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We developed a new bacterial expression system that utilizes a combination of attributes (low temperature, induction of an mRNA-specific endoribonuclease causing host cell growth arrest, and culture condensation) to facilitate stable, high level protein expression, almost 30% of total cellular protein, without background protein synthesis. ...
microbiology metabolism isolation & purification biosynthesis genetics 
Genes, Bacterial Recombinant Proteins Escherichia coli Genetic Vectors Endoribonucleases DNA-Binding Proteins Plasmids Selenomethionine Nitrogen Isotopes p-Fluorophenylalanine Escherichia coli Proteins Gene Expression Bioreactors 
17020876  
10.1074/jbc.M608806200  
37 (Last update: 05/27/2017 12:08:24pm)  
method  
J Biol Chem. 2006 Dec 8;281(49):37559-65. Epub 2006 Oct 4.