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NMR data collection and analysis protocol for high-throughput protein structure determination.

Liu G, Shen Y, Atreya HS, Parish D, Shao Y, Sukumaran DK, Xiao R, Yee A, Lemak A, Bhattacharya A, Acton TA, Arrowsmith CH, Montelione GT, Szyperski T,
Proceedings of the National Academy of Sciences of the United States of America (2005) 102(30):10487-92 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform NMR experiments for resonance assignment based on highly resolved 4D and 5D spectral information is acquired in conjunction with a single simultaneous 3D 15N,13C(aliphatic),13C(aromatic)-resolved [1H,1H]-NOESY spectrum providing 1H-1H upper distance limit constraints. ...
chemistry methods 
Models, Molecular Data Collection Protein Conformation Fourier Analysis Proteins Nuclear Magnetic Resonance, Biomolecular 
16027363  
10.1073/pnas.0504338102  
94 (Last update: 03/25/2017 11:18:51am)  
1XPV  
method  
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10487-92. Epub 2005 Jul 18.