|Proceedings of the National Academy of Sciences of the United States of America (2005) 102(30):10487-92|
|Northeast Structural Genomics Consortium|
(click to unfold)
A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform NMR experiments for resonance assignment based on highly resolved 4D and 5D spectral information is acquired in conjunction with a single simultaneous 3D 15N,13C(aliphatic),13C(aromatic)-resolved [1H,1H]-NOESY spectrum providing 1H-1H upper distance limit constraints. ...
The protocol was integrated with methodology for semiautomated data analysis and used to solve eight NMR protein structures of the Northeast Structural Genomics Consortium pipeline. The molecular masses of the hypothetical target proteins ranged from 9 to 20 kDa with an average of approximately 14 kDa. Between 1 and 9 days of instrument time were invested per structure, which is less than approximately 10-25% of the measurement time routinely required to date with conventional approaches. The protocol presented here effectively removes data collection as a bottleneck for high-throughput solution structure determination of proteins up to at least approximately 20 kDa, while concurrently providing spectra that are highly amenable to fast and robust analysis.
|chemistry methods |
|Models, Molecular Data Collection Protein Conformation Fourier Analysis Proteins Nuclear Magnetic Resonance, Biomolecular |
|93 (Last update: 07/15/2017 11:12:27am)|
|Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10487-92. Epub 2005 Jul 18.|