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Robotic cloning and protein production platform of the Northeast Structural Genomics Consortium.

Acton TB, Gunsalus KC, Xiao R, Ma LC, Aramini J, Baran MC, Chiang YW, Climent T, Cooper B, Denissova NG, Douglas SM, Everett JK, Ho CK, Macapagal D, Rajan PK, Shastry R, Shih LY, Swapna GV, Wilson M, Wu M, Gerstein M, Inouye M, Hunt JF, Montelione GT,
Methods in Enzymology (2005) 394(3):210-43 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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In this chapter we describe the core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) and outline the strategies used for producing high-quality protein samples using Escherichia coli host vectors. ...
methods genetics isolation & purification 
Computational Biology Magnetic Resonance Spectroscopy Robotics Cloning, Molecular Proteins Software Chromatography, Gel Protein Biosynthesis 
15808222  
10.1016/S0076-6879(05)94008-1  
104 (Last update: 03/18/2017 11:24:57am)  
Primer Prim’er – PCR Primer Design Software
 
method  
Methods Enzymol. 2005;394:210-43.