|Journal of Biological Chemistry (2005) 280(19):19213-20|
|Midwest Center for Structural GenomicsNortheast Structural Genomics Consortium|
(click to unfold)
A combination of structural, biochemical, and genetic studies in model organisms was used to infer a cellular role for the human protein (SBDS) responsible for Shwachman-Bodian-Diamond syndrome. ...
The crystal structure of the SBDS homologue in Archaeoglobus fulgidus, AF0491, revealed a three domain protein. The N-terminal domain, which harbors the majority of disease-linked mutations, has a novel three-dimensional fold. The central domain has the common winged helix-turn-helix motif, and the C-terminal domain shares structural homology with known RNA-binding domains. Proteomic analysis of the SBDS sequence homologue in Saccharomyces cerevisiae, YLR022C, revealed an association with over 20 proteins involved in ribosome biosynthesis. NMR structural genomics revealed another yeast protein, YHR087W, to be a structural homologue of the AF0491 N-terminal domain. Sequence analysis confirmed them as distant sequence homologues, therefore related by divergent evolution. Synthetic genetic array analysis of YHR087W revealed genetic interactions with proteins involved in RNA and rRNA processing including Mdm20/Nat3, Nsr1, and Npl3. Our observations, taken together with previous reports, support the conclusion that SBDS and its homologues play a role in RNA metabolism.
|metabolism chemistry physiology methods |
|Crystallography, X-Ray Acetyltransferases Nuclear Proteins Protein Structure, Tertiary Magnetic Resonance Spectroscopy RNA-Binding Proteins Protein Conformation RNA, Ribosomal Proteomics Protein Folding Protein Structure, Secondary Amino Acid Sequence Genomics Sequence Homology, Amino Acid Phylogeny Saccharomyces cerevisiae Proteins Molecular Sequence Data Saccharomyces cerevisiae Proteins Protein Binding RNA Amino Acid Motifs Archaeoglobus fulgidus Static Electricity |
|78 (Last update: 07/15/2017 11:17:09am)|
|J Biol Chem. 2005 May 13;280(19):19213-20. Epub 2005 Feb 8.|