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Cold-shock induced high-yield protein production in Escherichia coli.

Qing G, Ma LC, Khorchid A, Swapna GV, Mal TK, Takayama MM, Xia B, Phadtare S, Ke H, Acton T, Montelione GT, Ikura M, Inouye M,
Nature Biotechnology (2004) 22(7):877-82 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Overexpression of proteins in Escherichia coli at low temperature improves their solubility and stability. ...
chemistry genetics metabolism 
Escherichia coli Genetic Vectors Humans Molecular Sequence Data Promoter Regions, Genetic Protein Conformation Cloning, Molecular Isotope Labeling Animals Amino Acid Sequence Proteins Nuclear Magnetic Resonance, Biomolecular Bacterial Proteins Cold Temperature Protein Biosynthesis 
15195104  
10.1038/nbt984  
199 (Last update: 05/27/2017 11:38:17am)  
method  
Nat Biotechnol. 2004 Jul;22(7):877-82. Epub 2004 Jun 13.