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Cold-shock induced high-yield protein production in Escherichia coli.

Qing G, Ma LC, Khorchid A, Swapna GV, Mal TK, Takayama MM, Xia B, Phadtare S, Ke H, Acton T, Montelione GT, Ikura M, Inouye M,
Nature Biotechnology (2004) 22(7):877-82 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Overexpression of proteins in Escherichia coli at low temperature improves their solubility and stability. ...
metabolism chemistry genetics 
Humans Amino Acid Sequence Animals Molecular Sequence Data Proteins Escherichia coli Cloning, Molecular Protein Conformation Cold Temperature Bacterial Proteins Nuclear Magnetic Resonance, Biomolecular Isotope Labeling Genetic Vectors Protein Biosynthesis Promoter Regions, Genetic 
15195104  
10.1038/nbt984  
203 (Last update: 11/10/2018 2:23:13pm)  
method  
Nat Biotechnol. 2004 Jul;22(7):877-82. Epub 2004 Jun 13.