|Protein Science (2003) 12(12):2823-30|
|Northeast Structural Genomics Consortium|
(click to unfold)
We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. ...
The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.
|Models, Molecular Pyrococcus horikoshii Molecular Sequence Data Sequence Alignment Ribosomal Proteins Amino Acid Sequence Nuclear Magnetic Resonance, Biomolecular |
|12 (Last update: 07/22/2017 11:12:02am)|
|Protein Sci. 2003 Dec;12(12):2823-30.|