|Journal of the American Chemical Society (2003) 125(41):12382-3|
|Northeast Structural Genomics Consortium|
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The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. ...
Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
|Models, Molecular Peptides Protein Conformation Spectrometry, Mass, Electrospray Ionization Anti-Bacterial Agents Bacteriocins |
|146 (Last update: 02/16/2019 2:37:55pm)|
|J Am Chem Soc. 2003 Oct 15;125(41):12382-3.|