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Refolding out of guanidine hydrochloride is an effective approach for high-throughput structural studies of small proteins.

Maxwell KL, Bona D, Liu C, Arrowsmith CH, Edwards AM,
Protein Science (2003) 12(9):2073-80 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Low in vivo solubility of recombinant proteins expressed in Escherichia coli can seriously hinder the purification of structural samples for large-scale proteomic NMR and X-ray crystallography studies. ...
metabolism chemistry methods isolation & purification 
Crystallography, X-Ray Proteins Escherichia coli Proteomics Protein Conformation Protein Folding Recombinant Proteins Circular Dichroism Magnetic Resonance Spectroscopy Proteome Electrophoresis, Polyacrylamide Gel Biophysical Phenomena Biophysics Protein Denaturation Guanidine 
27 (Last update: 03/16/2019 1:54:04pm)  
Protein Sci. 2003 Sep;12(9):2073-80.