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Strategies for structural proteomics of prokaryotes: Quantifying the advantages of studying orthologous proteins and of using both NMR and X-ray crystallography approaches.

Savchenko A, Yee A, Khachatryan A, Skarina T, Evdokimova E, Pavlova M, Semesi A, Northey J, Beasley S, Lan N, Das R, Gerstein M, Arrowmith CH, Edwards AM,
Proteins (2003) 50(3):392-9 PublishedPSI:Phase 2  
Midwest Center for Structural GenomicsNortheast Structural Genomics Consortium

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Only about half of non-membrane-bound proteins encoded by either bacterial or archaeal genomes are soluble when expressed in Escherichia coli (Yee et al., Proc Natl Acad Sci USA 2002;99:1825-1830; Christendat et al., Prog Biophys Mol Biol 200;73:339-345). ...
chemistry methods genetics isolation & purification analysis 
Crystallography, X-Ray Proteomics Escherichia coli Proteins Cloning, Molecular Bacterial Proteins Nuclear Magnetic Resonance, Biomolecular Archaeal Proteins Gene Expression Proteome Thermotoga maritima Solubility 
12557182  
10.1002/prot.10282  
64 (Last update: 06/23/2018 12:02:16pm)  
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