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Strategies for structural proteomics of prokaryotes: Quantifying the advantages of studying orthologous proteins and of using both NMR and X-ray crystallography approaches.

Savchenko A, Yee A, Khachatryan A, Skarina T, Evdokimova E, Pavlova M, Semesi A, Northey J, Beasley S, Lan N, Das R, Gerstein M, Arrowmith CH, Edwards AM,
Proteins (2003) 50(3):392-9 PublishedPSI:Phase 2  
Midwest Center for Structural GenomicsNortheast Structural Genomics Consortium

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Only about half of non-membrane-bound proteins encoded by either bacterial or archaeal genomes are soluble when expressed in Escherichia coli (Yee et al., Proc Natl Acad Sci USA 2002;99:1825-1830; Christendat et al., Prog Biophys Mol Biol 200;73:339-345). ...
analysis methods genetics isolation & purification chemistry 
Crystallography, X-Ray Archaeal Proteins Proteome Solubility Cloning, Molecular Thermotoga maritima Proteomics Nuclear Magnetic Resonance, Biomolecular Bacterial Proteins Escherichia coli Proteins Gene Expression 
64 (Last update: 04/21/2018 11:57:00am)