|Acta Crystallographica Section D: Biological Crystallography (2003) 59(Pt 1):127-35|
|New York Structural Genomics Research Consortium|
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Yeast hypothetical protein YBL036C (SWISS-PROT P38197), initially thought to be a member of an 11-protein family, was selected for crystal structure determination since no structural or functional information was available. ...
The structure has been determined independently by MIR and MAD methods to 2.0 A resolution. The MAD structure was determined largely through automated model building. The protein folds as a TIM barrel beginning with a long N-terminal helix, in contrast to the classic triose phosphate isomerase (TIM) structure, which begins with a beta-strand. A cofactor, pyridoxal 5'-phosphate, is covalently bound near the C-terminal end of the barrel, the usual active site in TIM-barrel folds. A single-domain monomeric molecule, this yeast protein resembles the N-terminal domain of alanine racemase or ornithine decarboxylase, both of which are two-domain dimeric proteins. The yeast protein has been shown to have amino-acid racemase activity. Although selected as a member of a protein family having no obvious relationship to proteins of known structure, the protein fold turned out to be a well known and widely distributed fold. This points to the need for a more comprehensive base of structural information and better structure-modeling tools before the goal of structure prediction from amino-acid sequences can be realised. In this case, similarity to a known structure allowed inferences to be made about the structure and function of a widely distributed protein family.
|metabolism chemistry methods genetics |
|Crystallography, X-Ray Models, Molecular Ornithine Decarboxylase Alanine Racemase Pyridoxal Phosphate Databases, Protein Molecular Sequence Data Saccharomyces cerevisiae Triose-Phosphate Isomerase Fungal Proteins Protein Structure, Secondary Amino Acid Sequence Genomics Sequence Homology, Amino Acid |
|18 (Last update: 09/16/2017 11:45:50am)|
|Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):127-35. Epub 2002 Dec 19.|