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Protein production: feeding the crystallographers and NMR spectroscopists.

Edwards AM, Arrowsmith CH, Christendat D, Dharamsi A, Friesen JD, Greenblatt JF, Vedadi M,
Nature Structural & Molecular Biology (2000) 7 Suppl:970-2 PublishedPSI:Phase 2  
Northeast Structural Genomics Consortium

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Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. ...
isolation & purification chemistry biosynthesis genetics standards 
Crystallography, X-Ray Protein Structure, Tertiary Recombinant Fusion Proteins Robotics Cloning, Molecular Animals Quality Control Membrane Proteins Proteins Nuclear Magnetic Resonance, Biomolecular Gene Expression Protein Biosynthesis 
11104003  
10.1038/80751  
122 (Last update: 07/22/2017 11:48:54am)  
method  
Nat Struct Biol. 2000 Nov;7 Suppl:970-2.