|Neuron (2007) 54(4):511-33|
|Center for Structures of Membrane Proteins|
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Structural biology now plays a prominent role in addressing questions central to understanding how excitable cells function. ...
Although interest in the insights gained from the definition and dissection of macromolecular anatomy is high, many neurobiologists remain unfamiliar with the methods employed. This primer aims to help neurobiologists understand approaches for probing macromolecular structure and where the limits and challenges remain. Using examples of macromolecules with neurobiological importance, the review covers X-ray crystallography, electron microscopy (EM), small-angle X-ray scattering (SAXS), and nuclear magnetic resonance (NMR) and biophysical methods with which these approaches are often paired: isothermal titration calorimetry (ITC), equilibrium analytical ultracentifugation, and molecular dynamics (MD).
|methods standards |
|Crystallography, X-Ray Humans Animals Models, Molecular Computational Biology Structure-Activity Relationship Molecular Structure Magnetic Resonance Spectroscopy Microscopy, Electron Neurobiology |
|31 (Last update: 01/19/2019 8:33:32pm)|
|Neuron. 2007 May 24;54(4):511-33.|