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Cell-free protein synthesis technology in NMR high-throughput structure determination.

Makino S, Goren MA, Fox BG, Markley JL,
Methods in Molecular Biology (2010) 607:127-47 PublishedPSI:Phase 2  
Center for Eukaryotic Structural Genomics

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This chapter describes the current implementation of the cell-free translation platform developed at the Center for Eukaryotic Structural Genomics (CESG) and practical aspects of the production of stable isotope-labeled eukaryotic proteins for NMR structure determination. ...
metabolism chemistry methods genetics biosynthesis embryology 
Histidine Protein Conformation Recombinant Proteins Magnetic Resonance Spectroscopy Protein Engineering Isotope Labeling Protein Biosynthesis Oligopeptides Cell-Free System Triticum Seeds High-Throughput Screening Assays 
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