Wladek Minor, Ph.D.

 Professor of Molecular Physiology and Biological Physics

- Office:  (434) 243-6865
- Lab:  (434) 924-2948
- Lab 2 (coffee room desks):  (434) 243-0033
- Lab 3 (former front office):  (434) 243-6853
- Fax:  (434) 243-2981
- E-mail:  wladek@iwonka.med.virginia.edu
- Directions to the laboratory (in PDF format)
    Department of Molecular Physiology
and Biological Physics
University of Virginia
1340 Jefferson Park Avenue,
Jordan Hall
Room 4223
Charlottesville, VA 22908
    Department of Molecular Physiology
and Biological Physics
University of Virginia
PO Box 800736
Charlottesville, VA 22908-073

Research Interests:

lipoxygenase

Our laboratory studies macromolecular structure with an aim toward in-depth understanding of structure-function relationships. X-ray diffraction analysis is our primary research tool, but we also employ other physical and biochemical methods of analysis. The program emphasizes two broad themes; crystallographic studies on molecules of immediate interest, and methodology development. Most of the macromolecules that we have under study relate to one or more of a few broad biological areas: cellular signal transduction and metalloproteins. The same systems have been chosen as subjects for methodology development. The methodology development includes the development of various crystallographic tools that create the HKL Package.

Another research area is high-throughput crystallography and structural genomics. Our lab is involved in a number of large, biomedically oriented projects that will revolutionize biomedical research in this decade. We are a member of the Midwest Center for Structural Genomics and the New York Structural Genomics Research Consortium (both centers of the NIH Protein Structure Initiative), and the Center for Structural Genomics of Infectious Disease (a project of the NIAID). We are also a part of the Enzyme Function Initiative (an NIH Glue Grant). We develop widely used methodology that is used in thousands of structural biology laboratories around the world. We collaborate with many synchrotron beamlines, in particular, with the Structural Biology Center at the Advanced Photon Source, and with many individual laboraries.

electron density

These collaborations have been exceptionally fruitful, resulting in joint publications with researchers from 79 different institutions from around the world. See an interactive map of the geographical distribution of these institutions.

The lab is well equipped to facilitate large scale protein purification, crystallization, biophysical characterization and detection of protein/protein or protein/small molecule interactions.

Web Services

  Check My Metal -- A web server for the validation of metal binding sites in macromolecules.
  MgRNA -- Classification of Mg2+ binding sites in RNA crystal structures
  Fitmunk -- Automatic modeling of side-chain conformations
  The Integrated Resource for Reproducibility in Macromolecular Crystallography -- Diffraction Experiment Archiver
Thumbnail Gallery

Recent SAD/MAD Structures

All structures are available with links

5bxy
2015-06-09
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5bp9
2015-05-27
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5bp7
2015-05-27
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4znz
2015-05-05
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4yyc
2015-03-23
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4wji
2014-09-30
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4wed
2014-09-09
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4wcz
2014-09-05
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4wbt
2014-09-03
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4u12
2014-07-14
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4pmj
2014-05-21
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4omr
2014-01-27
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4olq
2014-01-24
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4o9k
2014-01-02
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4nek
2013-10-29
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4ne4
2013-10-28
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4nd9
2013-10-25
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4n18
2013-10-03
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4mou
2013-09-12
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4mdc
2013-08-22
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4jyl
2013-03-29
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4jyj
2013-03-29
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4jxu
2013-03-28
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4jxq
2013-03-28
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4jwv
2013-03-27
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4jsb
2013-03-22
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4iqg
2013-01-11
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4igv
2012-12-18
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4he5
2012-10-03
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4gqa
2012-08-22
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4euy
2012-04-25
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3v48
2011-12-14
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3v4e
2011-12-14
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3v4d
2011-12-14
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3v0r
2011-12-08
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3uwd
2011-12-01
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3udu
2011-11-01
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3udo
2011-11-01
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3uec
2011-10-30
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3tnj
2011-09-01
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3tl2
2011-08-29
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3t7b
2011-07-29
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3t5p
2011-07-27
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3sks
2011-06-23
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3rao
2011-03-28
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3qtb
2011-02-22
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3qtd
2011-02-22
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3qsl
2011-02-21
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3p7m
2010-10-12
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3ot1
2010-09-16
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3os6
2010-09-08
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3op4
2010-08-31
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3o4f
2010-07-27
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3ni7
2010-06-15
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3n73
2010-05-26
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3n0s
2010-05-14
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3m3p
2010-03-09
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3lqy
2010-02-10
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3loc
2010-02-03
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3kxr
2009-12-15
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3kzp
2009-12-08
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3kzl
2009-12-08
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3kkd
2009-11-05
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3k3s
2009-10-04
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3iii
2009-08-01
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3ibz
2009-07-17
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3icc
2009-07-17
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3i4p
2009-07-02
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3hfr
2009-05-12
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3h5q
2009-04-22
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3h3m
2009-04-16
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3fzv
2009-01-26
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3ftt
2009-01-13
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3elk
2008-09-22
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3eef
2008-09-04
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3dm8
2008-06-30
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3dcl
2008-06-03
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3dca
2008-06-03
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3cnv
2008-03-26
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3cni
2008-03-25
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2qnu
2007-07-19
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2qmo
2007-07-16
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2q24
2007-05-25
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2pz9
2007-05-17
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2pfs
2007-04-05
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2pd0
2007-03-30
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2pc6
2007-03-29
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2paq
2007-03-27
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2ouf
2007-02-10
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2ofy
2007-01-04
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2o9x
2006-12-14
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2o8n
2006-12-12
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2o0y
2006-11-28
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2np3
2006-10-26
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2np5
2006-10-26
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2id3
2006-09-14
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2iai
2006-09-08
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2ia2
2006-09-06
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2i9c
2006-09-05
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2i8e
2006-09-01
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2i6c
2006-08-28
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2hr3
2006-07-19
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2dg2
2006-03-08
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2g7u
2006-03-01
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2g3a
2006-02-17
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2g3b
2006-02-17
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2fgc
2005-12-21
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2ffs
2005-12-20
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2fex
2005-12-16
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2fef
2005-12-15
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2fdo
2005-12-14
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2f96
2005-12-05
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2av9
2005-08-29
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1z77
2005-03-24
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All structures are available with links

Selected Lab Alumni

 Maks Chruszcz, Associate Professor, University of South Carolina, Columbia, SC
 Krzysztof Lewinski, Professor, Jagellonian University, Krakow, Poland
 Hai Bin Luo, Professor, Research Center for Drug Discovery, Sun Yat-Sen University, Guangzhou, China
 Diana Tomchick, Professor, UT Southwestern Medical Center, Dallas, TX
 James Spencer, Senior Lecturer in Microbial Pathogenesis, University of Bristol, Bristol, UK
 Ekaterina Filippova, Research Associate, Northwestern University, Chicago, IL
 Katarzyna Koclega, Wroclaw Research Centre EIT+, Wroclaw, Poland
 Janusz Petkowski, Research Associate, ETH Zurich, Switzerland
 Aleksandra Knapik, Graduate Student, Department of Cytobiochemistry, University of Lodz
 Alexandra Kos (Zalcman), Nexus Telecom, Zurich, Switzerland
 Robert Nicholls, Graduate Student, University of York, York, UK
 Haijun Qiu, Database Administrator, The Children's Hospital of Philadelphia, Philadelphia, PA
 John Raynor, Structural Genomics Consortium, Oxford University, Oxford, UK
 Pawel Sledz, Gates Scholar, University of Cambridge, Cambridge, UK
 Shuren Wang, Technical Data Manager, OpenQ Inc., Charlottesville, VA
 Arjun Dayal, Co-founder, Monte Carlo Software LLC
 Marcin Domagalski, Postdoctoral Fellow, Swiss Institute of Bioinformatics
 Karolina Majorek, Postdoctoral Research Scientist at The Beatson Institute for Cancer Research, Glasgow, Scotland
 Ewa Niedzialkowska, Postdoctoral Researcher, Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences
 Przemek Porebski, Postdoctoral Researcher, Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences
 Matthew Zimmerman, Data scientist at CCRi
A full list of Minor Lab alumni is also available

Selected Publications

  1. Zimmerman MD, Proudfoot M, Yakunin A, Minor W  (2008)
    Structural Insight into the Mechanism of Substrate Specificity and Catalytic Activity of an HD-Domain Phosphohydrolase: The 5'-Deoxyribonucleotidase YfbR from Escherichia coli.
    Journal of Molecular Biology 378: 215-26.  Times cited: 37.  [Pub Med ID: 18353368] [Pub Med Central ID: 2504004]


  2. Kirillova O, Chruszcz M, Shumilin IA, Skarina T, Gorodichtchenskaia E, Cymborowski M, Savchenko A, Edwards A, Minor W  (2007)
    An Extremely SAD Case: Structure of a Putative Redox-Enzyme Maturation Protein from Archaeoglobus Fulgidus at 3.4 Angstrom Resolution.
    Acta Crystallographica Section D: Biological Crystallography 63: 348-54.  Times cited: 22.  [Pub Med ID: 17327672]


  3. Minor W, Cymborowski M, Otwinowski Z, Chruszcz M  (2006)
    HKL-3000: The Integration of Data Reduction and Structure Solution--From Diffraction Images to an Initial Model in Minutes.
    Acta Crystallographica Section D: Biological Crystallography 62: 859-66.  Times cited: 564.  [Pub Med ID: 16855301]


  4. Otwinowski Z, Borek D, Majewski W, Minor W  (2003)
    Multiparametric scaling of diffraction intensities.
    Acta Crystallographica. Section A: Foundations of Crystallography 59: 228-34.  Times cited: 522.  [Pub Med ID: 12714773]


  5. Otwinowski Z, Minor W  (1997)
    Processing of X-ray diffraction data collected in oscillation mode.
    Methods in Enzymology 276: 307-326.  Times cited: 30788.  


  6. Minor W, Steczko J, Stec B, Otwinowski Z, Bolin JT, Walter R, Axelrod B  (1996)
    Crystal structure of soybean lipoxygenase L-l at 1.4 angstrom resolution.
    Biochemistry 35: 10687-701.  Times cited: 334.  [Pub Med ID: 8718858]


  7. Minor W, Steczko J, Bolin JT, Otwinowski Z, Axelrod B  (1993)
    Crystallographic Determination Of The Active-Site Iron And Its Ligands In Soybean Lipoxygenase L-1.
    Biochemistry 32: 6320-3.  Times cited: 139.  [Pub Med ID: 8518276]


More publications with external links are available.

Last modified: Mon March 21, 2016 12:40:46 PM
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